DNA hypomethylation of individual sequences in aborted cloned bovine fetuses.
نویسندگان
چکیده
Cloned bovines have a much higher abortion rate than those derived in vivo. Available evidence indicates that inappropriate epigenetic reprogramming of donor nuclei is the primary cause of cloning failure. To gain a better understanding of the DNA methylation changes associated with the high abortion rate of cloned bovines, we examined the DNA methylation status of a repeated sequence (satellite I) and the promoter regions of two single-copy genes (interleukin 3/cytokeratin) in aborted cloned fetuses, aborted fetuses derived from artificial insemination (AI), cloned adults and AI adults by bisulfite sequencing and restriction enzyme analysis. Two of four aborted cloned fetuses show very low methylation levels in the two single-copy gene promoter regions. One of the two fetuses also showed undermethylated status in the satellite I sequence. The other two aborted cloned fetuses have similar methylation levels to those of aborted AI fetuses. However, no difference in methylation was observed between cloned adults and AI adults. Our results demonstrate for the first time the undermethylated status of individual sequences in aborted cloned fetuses. These findings suggest that aberrant DNA methylation may contribute to the developmental failure of cloned bovine fetuses.
منابع مشابه
Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos
Cloned pigs generated by somatic cell nuclear transfer (SCNT) show a greater ratio of early abortion during mid-gestation than normal controls. X-linked genes have been demonstrated to be important for the development of cloned embryos. To determine the relationship between the expression of X-linked genes and abortion of cloned porcine fetuses, the expression of X-linked genes were investigate...
متن کاملMolecular detection of Toxoplasma gondii infection in aborted fetuses in sheep in Khorasan Razavi province, Iran
Background: Toxoplasma gondii is a significant cause of abortion in sheep and goats in the world. Toxoplasmosis is caused reproduction disorders such as fetal resorption, early embryonic death, mummification, abortion, stillbirth, neonatal and fetal death in sheep . Objectives: The aim of this study was to detect T. gondii infection in ovine aborted fetuses in Khorasan Razavi province. Methods:...
متن کاملEstimating the rate of transplacental transmission of Neospora caninum to aborted fetuses in seropositive dams in Mashhad area, Iran
BACKGROUND: Neospora caninum is an apicomplexan protozoa,which causes abortion in cattle worldwide. OBJECTIVES:The present study was designed to estimate the rate oftransplacental transmission of N.caninum to aborted fetuses inseropositive dams in dairy farms in Mashhad area, Iran.METHODS: Two hundred blood samples of dam with abortedbovine fetuses were collected from dairy farms from 2008 to20...
متن کاملMolecular Testing for Toxoplasma Diagnosis in Aborted Fetuses- Taleghani Maternity Hospital- Arak- Iran
Background: The diagnosis of toxoplasmosis is most critical in pregnant women who acquire infection during gestation and also in fetuses and newborns who are congenitally infected. This study described the performance of molecular and confirmatory serologic testing for toxoplasma infection in the tissues of human spontaneous aborted fetuses and their mothers' blood. Materials and Methods...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Frontiers in bioscience : a journal and virtual library
دوره 10 شماره
صفحات -
تاریخ انتشار 2005